鈣通量分析是篩選G蛋白偶聯(lián)受體（GPCR）藥品發(fā)現(xiàn)的方法。該比值鈣測(cè)定試劑盒允許均勻測(cè)量由G蛋白偶聯(lián)受體或鈣通道引起的細(xì)胞內(nèi)鈣變化。表達(dá)通過(guò)鈣傳遞信號(hào)的GPCR的細(xì)胞被Fura-2AM預(yù)加載，F(xiàn)ura-2AM可以穿過(guò)細(xì)胞膜。一旦進(jìn)入細(xì)胞內(nèi)，親脂封閉基團(tuán)Fura-2AM被酯酶裂解，導(dǎo)致帶負(fù)電荷的熒光染料留在細(xì)胞內(nèi)，其熒光波長(zhǎng)在與鈣結(jié)合時(shí)藍(lán)移。當(dāng)細(xì)胞受到激動(dòng)劑刺激時(shí)，受體發(fā)出細(xì)胞內(nèi)鈣離子釋放的信號(hào)，從而大大提高Fura-2在短波處的熒光強(qiáng)度。Fura-2的比色特性使該試劑盒成為比單波長(zhǎng)Fluo-4測(cè)定細(xì)胞鈣濃度的理想工具。通過(guò)單次添加，該分析很容易在高透性環(huán)境中進(jìn)行。該方法可用于96孔或384孔微孔板，且易于自動(dòng)化。百螢生物是AAT Bioquest的中國(guó)代理商，為您提供的Screen Quest 免洗Fura-2鈣檢測(cè)試劑盒

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